Table 4. Non-synonymous variations in COLEC10 and COLEC11.

Variation	Region	PolyPhen-2	Blosum62	SIFT	Align-GVGD	PhD-SNP	Consurf
COLEC10 Glu78Asp (rs150828850)	Exon 3	Benign	2	1	C0	Neutral (7)	Variable(3)
COLEC10 Arg125Trp (rs149331285)	Exon 5	Probably damaging	-3	0	C65	Disease (3)	Conserved (9)
COLEC11 His219Arg (rs7567833)	Exon 7	Benign	0	0.462	29	Neutral (3)	Variable(3)

PolyPhen-2 classifies qualitatively a mutation as benign, possibly damaging, or probably damaging. BLOSUM62 matrix values range from 4 (more likely) to -4 (less likely substitutions). SIFT values >0.05 indicate substitutions with little effect on protein function, while values <0.05 point out possible deleterious substitutions. Align-GVGD categorizes a mutation in seven graded classifiers from less likely (class C0) to most likely to interfere with protein function (class C65). PhD-SNP produces a binary prediction (neutral vs disease-related polymorphism), together with the reliability score of the prediction (0–9). Consurf position-specific conservation scores are divided in 9 grades, from the most variable (grade 1) to the most conserved positions (grade 9).