Table 3. Confirmation of the transctiptomic data (RNA-seq) by Quantitative Real-time PCR (Q-PCR.
| cv. Lahontan | |||
|---|---|---|---|
| M. truncatula ID | RNA-seq | Q-PCR | Description |
| Medtr1g028720.1 | -2.97 | -3.14 | |NB-ARC domain disease resistance protein |
| Medtr3g027330.1 | 1.80 | -2.07 | |receptor-like protein| |
| Medtr5g036290.1 | 2.65 | 1.51 | |matrixin family protein |
| Medtr6g075870.1 | 2.86 | 1.98 | |disease resistance protein (TIR-NBS-LRR class) |
| Medtr7g065160.1 | -3.85 | -0.38 | |Lipid transfer protein |
| Medtr1g115135.1 | -2.32 | -1.31 | |calcium-binding EF hand-like protein |
| Medtr3g023030.1 | 1.58 | 0.59 | |LRR and NB-ARC domain disease resistance protein |
| Medtr1g027290.1 | 2.57 | 1.84 | |flavonolsynthase/flavanone3-hydroxylase |
| Medtr7g068350.1 | 2.76 | 1.55 | |transmembrane protein, putative |
| Medtr0277s0020.3 | -2.54 | 0.51 | |disease resistance protein (TIR-NBS-LRR class) |
| Medtr7g105720.1 | -2.71 | -1.33 | |hypothetical protein |
| Medtr3g056585.1 | -2.17 | -2.01 | |LRR and NB-ARC domain disease resistance protein* |
| Medtr5g087320.1 | -2.14 | -2.05 | |receptor-like protein* |
| Medtr3g052720.1 | 3.82 | -0.67 | |organelle transcript processing protein, putative |
| Medtr7g082460.1 | 2.83 | -0.49 | |receptor-like kinase |
| Medtr7g026140.1 | 3.05 | 1.66 | |heat shock cognate70kDaprotein |
| Medtr8g015830.1 | 4.90 | 1.15 | |F-box/FBD-like domain protein, putative |
| Medtr5g081480.1 | 4.18 | 2.41 | |hypothetical protein |
| Medtr6g015490.1 | 3.59 | 1.41 | |disease resistance protein (TIR-NBS-LRR class) |
| Medtr4g105540.1 | -2.78 | -1.17 | |cysteine-rich receptor-kinase-like protein |
| Medtr8g007125.1 | -3.87 | -4.29 | |patatin-like phospholipase |
| Medtr1g052470.1 | -4.64 | -12.45 | |transcription factorbHLH122-like protein |
Bold font: gene expression data consistent between RNA-seq and qPCR;
*, two putative R-genes up-regulated during infection in cv. Moapa 69 and repressed in cv. Lahontan.