Figure 2.

Different outcome of 12-week kidney grafts inside the LNs. (A): Immunofluorescence staining of Pdpl, CD31, CIV, CK8, and EPO (red) on sections of 12-week kidney graft (donor cells GFP⁺, green). Nuclei were counterstained using Hoechst (blue). (B): Whole-mount LN and H&E staining showing cysts inside a 12-week kidney graft (left panels) and cartoon depicting nephron structure and origin of cysts from PCT or tDLLH (right panel). (C): Detail of cyst 1 epithelium stained with H&E, PAS, TRI, PSR, GFP, BrdU, AQP1, and NKCC2 (left panels), and of cysts 2 and 3 epithelium stained with H&E, PAS, TRI, PSR, GFP, BrdU, AQP1 and 2 (right panels, yellow arrows indicate vacuoles). 3-Amino-9-ethylcarbazole (brown staining) was used to identify targets by immunohistochemistry. (D): Details of proteinaceous material found inside cyst 1 (left panel) and of round globules found inside cysts 2 and 3 (right panel) after H&E staining. (E): Pictures of urinary crystals found inside cyst 1. (F): Blood urea nitrogen levels in serum and LN fluid of a transplanted (16 weeks KLN) versus a control mouse. Abbreviations: AQP1, aquaporin 1; BrdU, bromodeoxyuridine; BUN, blood urea nitrogen; CIV, collagen IV; CK8, cytokeratin-8; CTRL, control; EPO, erythropoietin; GFP, green fluorescent protein; H&E, hematoxylin and eosin; KLN, kidney lymph node; LN, lymph node; NKCC2, sodium-potassium-chloride transporter 2; PAS, periodic acid-Schiff; PCT, proximal convoluted tubule; Pdpl, podoplanin; PSR, Picrosirius red; tDLLH, thin descending limb of Henle’s loop; TRI, Masson’s trichrome; wks, weeks.