Figure 6.

The effect of p38 inhibition on HIF-1α protein (A) and VEGFA and GLUT3 mRNA (B) in first trimester placental explants subjected to 2 or 21% O₂ ± 1 mM H₂O₂ for 6 h. (A) Lysates from first trimester explants cultured with or without the p38 inhibitor PD169316 (p38i; 10 µM) were immunoblotted with HIF-1α antibody. Poncaeu S staining served to normalize gel loading. Normalized results (±SEM) are plotted, expressing T₀ samples as 100%. (B) RNA was isolated and relative levels of VEGFA and GLUT3 mRNA were detected using quantitative real-time RT–PCR. VEGFA and GLUT3 mRNA levels were normalized to the 18S RNA levels. Different letters indicate groups that are significantly different (P < 0.05; one-way ANOVA + Student–Newman–Keuls test).