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. 2014 Nov 11;21(3):296–308. doi: 10.1093/molehr/gau105

Figure 7.

Figure 7

Immunoprecipitation experiment to evaluate the role of phosphorylation in HIF-1α regulation. Two samples from the same placenta (10 + 3 weeks) were snap frozen (T0) or cultured under 2% O2 in the presence of 1 mM H2O2 for 6 h. Lysates were immunoprecipitated with an antibody against phospho-Ser/Thr/Tyr, western blots were run, probed with anti-HIF-1α and re-probed with anti-phospho-Ser/Thr/Tyr. HIF-1α was only detected on the P-Ser/Thr/Tyr-coated beads from the cultured placenta.