Figure 1. Expression of RGS^e-βgl-D^d transgenes is restricted to hepatocytes directly surrounding the central vein in adult liver.

The rat glutamine synthetase upstream enhancer centered at −2.2 kb (RGS^e) was amplified as a 400 bp fragment and fused to βgl-D^d. Offspring of two different transgenic founders containing RGS^e-βgl-D^d were sacrified at ~8 weeks of age. Frozen sections were prepared and stained with a monoclonal FITC-anti-D^d antibody. D^d expression was restricted to hepatocytes directly surrounding the central veins. The same pattern of staining was observed with lines from two other RGS^e-βgl-D^d founder mice (data not shown). Magnification, 20X.