Table I.

Activity of enzymes recombinantly expressed in two expression systems

Protein	Pichia pastoris (mU mL−1)	Hi5 insect cells (mU mL−1)
DmFDL	88	n/d
DmFDLa	110	2500
AmFDL	150	250
CeHEX-2	2100	n/d
CeHEX-3	240	n/d
CeHEX-4	870	n/d

Units were calculated based on the pNP-β-GlcNAc for insect and on pNP-β-GalNAc for the Caenorhabditis enzymes. Assays were performed at the pH optimum of the respective enzymes. DmFDL assays were performed at 30°C, whereas all other enzymes were tested at 37°C. n/d, not determined.

^aP. pastoris codon-optimized protein.