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. 2015 Feb 17;6:6255. doi: 10.1038/ncomms7255

Figure 4. GP2 promotes the endocytotic uptake of L-PTC.

Figure 4

MDCK I monolayers were transiently transfected with mGP2, and seeded on transwells. Monolayers were apically treated with L-PTC (200 nM) for 40 min at 4 °C (upper panels) or 1 h at 37 °C (lower panels). L-PTC was labelled with anti-L-PTC antibody and Alexa Fluor 488-labelled anti-rabbit IgG antibody (green), and GP2 was labelled with anti-GP2 antibody and Cy3-labelled anti-rat IgG antibody (red). Endocytotic vesicles of L-PTC in GP2-negative (GP2−) or GP2-positive (GP2+) cells were counted (GP2− cells, 72 cells; GP2+ cells, 35 cells), and the data are expressed as total L-PTC vesicles per number of cells. Error bars indicate s.d. Statistical analyses were performed with Student’s t-test. L-PTC co-localized with GP2 in GP2+ cells (arrows). Scale bar, 10 μm. Data are representative of two independent experiments.