Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Feb 17;6:6255. doi: 10.1038/ncomms7255

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a,b) Alexa Fluor 568-labelled HA subcomponents or reconstituted HA (Alexa Fluor 568-labelled HA2/HA3 or HA1/Alexa Fluor 568-labelled HA2/HA3; red) were injected into ligated mouse intestinal loops and incubated for 2 h; FAE regions were stained with FITC-labelled UEA-1 (green). (c) Recombinant Fc proteins (mGP2 and hGP2) were incubated with Strep-Tactin Superflow agarose pre-bound to HA1, HA3, HA2/HA3 core complex or HA (HA1/HA2/HA3). HA-bound proteins were analyzed by immunoblotting using HRP-labelled anti-human IgG antibody. (d) Reconstituted WT HA (HA1/Alexa Fluor 568-labelled HA2/HA3), mutant HA complex harbouring mutant HA1 (N285A, HA1N285A/Alexa Fluor 568-labelled HA2/HA3), mutant HA complex harbouring mutant HA3 (R528A, HA1/Alexa Fluor 568-labelled HA2/HA3R528A) and mutant HA complex harbouring mutant HA1 and HA3 (N285A/R528A, HA1N285A/Alexa Fluor 568-labelled HA2/HA3R528A; red) were injected into ligated mouse intestinal loops and incubated for 2 h; FAE regions were stained with FITC-labelled UEA-1 (green). (e) Recombinant mGP2-Fc protein was incubated with Strep-Tactin Superflow agarose pre-bound to HA (WT, N285A, R528A or N285A/R528A). HA-bound proteins were analyzed by immunoblotting using HRP-labelled anti-human IgG antibody. (f) GST–mGP2 and GST were incubated with Strep-Tactin Superflow agarose pre-bound to HA (WT). HA-bound proteins were analyzed by immunoblotting using anti-GST antibody and HRP-labelled anti-rabbit IgG antibody. Scale bar, 10 μm (a,b,d). Data are representative of two (a,d) or three (b,c,e,f) independent experiments.