Skip to main content
. 2014 Dec 4;10(2):401–412. doi: 10.1021/cb5007618

Figure 6.

Figure 6

Estimation of anti-GAD polyclonal antibody fraction that recognizes 1. NOR9 serum was depleted of antibodies that bound 1 by passing 100 μL of 500 μg mL–1 serum over a TentaGel bead column displaying immobilized 1. As a control, serum was “mock depleted” by passing 500 μg mL–1 serum over a TentaGel column containing 3. A total of 50 μL of the resulting serum was incubated with GAD65-immobilized TentaGel beads, probed with Alexa Fluor 647 antimouse IgG, and analyzed by flow cytometry. Data reported are an average of three replicates ± s.d.