. 2014 Dec 4;10(2):401–412. doi: 10.1021/cb5007618

Table 2. Incidence of NOD and Swiss IgG Antibody Binding to GAD65 Immobilized on TentaGel Beads Determined Using a Flow Cytometry-Based Assay^a^,^b (40 Mice Were Tested: Swiss 1–20 and NOD 1–20).

mouse ID	Swiss	NOD
1		+
2		+
3		+
4		+
5		+
6		+
7
8
9		+
10		+
11
12		+
13		+
14		+
15		+
16		+
17
18		+
19		+
20		+

IgG binding conditions: 500 μg mL^–1 serum total protein in 50 μL of PBS containing 0.5% BSA, 0.05% Tween 20 was incubated with the beads containing immobilized GAD65-GST fusion protein for 2 h at RT.

“+” indicates that the MFI was higher than the three-sigma value of 20 000 fluorescence units for either one of two serum samples collected prior to age 8.

Table 2. Incidence of NOD and Swiss IgG Antibody Binding to GAD65 Immobilized on TentaGel Beads Determined Using a Flow Cytometry-Based Assaya,b (40 Mice Were Tested: Swiss 1–20 and NOD 1–20).

Table 2. Incidence of NOD and Swiss IgG Antibody Binding to GAD65 Immobilized on TentaGel Beads Determined Using a Flow Cytometry-Based Assay^a^,^b (40 Mice Were Tested: Swiss 1–20 and NOD 1–20).