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. 2012 May 15;7(14):1064–1072. doi: 10.3969/j.issn.1673-5374.2012.14.004

Figure 1.

Figure 1

Validation of luciferase as a suitable reference for real-time RT-PCR.

Titration curve showing the decrease in cycle threshold for luciferase as the number of mRNA transcripts added at the RNA isolation step is increased. Amplifications were performed in triplicate for each initial concentration; the value for each of these amplifications is shown as a hollow box.

The efficiency of the amplification calculated using the equation E = 10-1/m -1, where m is the slope of the fitted line-demonstrates that with each cycle, roughly 100% of the mRNA transcript is copied.