Skip to main content
. 2015 Feb 24;10(2):e0117769. doi: 10.1371/journal.pone.0117769

Fig 1. Treatment of ovaries with MHY1485 increased phosphorylation of mTOR pathway proteins and promoted secondary follicle development in vitro.

Fig 1

A) Treatment of ovaries with MHY1485 increased phosphorylation of mTOR as well as S6K1 and rpS6. Ovaries from day 10 mice were treated with MHY1485 for 3h before immunoblotting. Blotting figures for each immunoblotting test were cropped from full-length blots that are presented in S1 Fig. B) Ovarian weight changes. Paired ovaries from day 10 mice were incubated with MHY1485 with media changes at day 2 of culture. At the end of 4 days of incubation, ovaries were fixed before weighing, followed by histological analyses. Numbers in parentheses denote number of ovaries used. * P<0.05. C) Ovarian histology; bars: 100 um. D) Follicle dynamics. N = 5. * P<0.05.