Fig 3. Treatment with MHY1485 and subsequent grafting allowed the derivation of mature oocytes and healthy offspring.

A) Early embryonic development of oocytes after mTOR activator treatment. Ovaries were treated with MHY1485 for 2 days to activate follicles, followed by grafting into hosts for 5 days. Hosts were then treated with eCG and hCG. At 12h after hCG injection, mature oocytes were obtained and fertilized with sperm before culturing for 4 days. B) Percentage of oocytes developed into each embryonic stage. Early embryonic development for mice at 25 days of age served as controls. N = 30. C) Some 2-cell stage embryos were transferred into pseudopregnant hosts and pups were delivered.