Table II.
CEP290 primers for genomic QRT-PCR
| Exons | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) |
|---|---|---|
| 5′-UTR | CGGTCGTGAACTGTAGGCTCT | GCTGCTAGGCGACACCATC |
| 2 | GGTGGAGCACAGTGAAAGAATTC | TCTGCCAGTTCTTCTTGACGG |
| 3 | GTTGCTTTTCTGTTTACTTAGGTGGA | AATTCTGAAAAGGTGTATCACATTTT |
| 4 | CTCAAGAAGTGGAGCTGGCTTT | TTTTTCCAAGGTGCTTACCAAATT |
| 5 | TTGTATTCCCTAGGATGTAATTGTCATT | TACAATCATCCTTATAATTTTTCCAGC |
| 6 | TTACGTAATGAAATTTGCCAACTTG | CTCCAACTCCTTTTCCATGTCC |
| 7 | GAGGAGGCAGAAAATGAAAACAG | AAGTACCTTTGTTGAACCACCACA |
| 8 | ACCGTTTTCCTGTCCTTTTCTG | AAATTCCCAAGATTTCACCACACT |
| 9 | AAGACAGTGACTACCGATCACAGTTG | GCCATTTTACCTGAATTTCATCAAG |
| 10 | TGAAAGCTATTGTGCATCAGACAG | TTCTTACTTGAAGTTGATAATGATCG |
| 11 | GTCTTTTAGGTGCAGGAGCTTACAG | TGCATTGACAGCTACCATAATTGG |
| 12 | AAAGATGATGAAATTATTGAGTATCAG | ATCAGCATCAAGCTGAGCATTC |
| 13 | TGCTCACCGAACAAGTAGAACAA | GGAGCTCATTTTTCAAATCTTCAATA |
| 14 | GCTTCAACCCTTTCTCAACAGAC | CTGTTCTCTCAGCCTCTTTAGTTTTC |
| 15 | TCGAGAGATTGAAATATTAACAAAGG | AGTGCCTCATTTTCATCAAGGAA |
| 16 | GCTTTCTATTGTAGGCCTTGAACC | TGCTGCTGTTTTAAGTGTTTGCTA |
| 17 | AAGAAGTGCAACTTCAGGTATACTCAG | TTTCATATCCAGACAACTCACTTATC |
| 18 | CACTGAGGACCTGAACCTAACTGA | TTGAGGCTCAATAAATCCAATTTTC |
| 19 | GAAAGGAGTAGGACAGTGATAGCCA | AAACAGAGAATGTGTTAACGCCCT |
| 20 | GCAACTTGAAGAAGGTATGAAAGAAAT | GATGTTTCTCCTCCTTTAACATCAGG |
| 21 | GCTAGGCTATAGAATCAAAGAATGCA | CGGTAAGCTGATCAACTTGGG |
| 22 | TCTGTCAATTTGTCTTTCTTTGGG | TTTCAAGATGGTCTATCTGGAAAAAA |
| 23 | TTTGCTGTAATTCGTCATCAACAA | ACTTTCACAATTAACAAGAATATACT |
| 24 | TAAAAGAGGAAAAGAGAAAACTTGAGG | TTATAGCATCTTGTTGGACTTGATCC |
| 25 | CAACCTTAGTAGAATTGGAGCGACA | CTTCAGCCTCCATTGACAACAA |
| 26 | ATGGCCATTTTCAAGATTGCA | TTTATTAGCCAGTTCTAGTTCAGACA |
| 27 | CCTTTATTCAGTGTGAAAACATCTCC | GTTTTTCCTTGGTAATCTCCAGTTCT |
| 28 | AATTAAATGAAAGGCAGCGGG | GTTCCTCCATTTGCTTTAACGAA |
| 29 | TCAATTTGGATGCACAGAAGGT | CAGCATCACTTACTGCCTTGCT |
| 30 | CACAACAACAATCTAGGGACAAGG | GAGCCAATACTGCACATACCTGAT |
| 31 | ATGGAGGCCTACAACTTGCG | TCTTCCCTCCAAACGAGCAT |
| 32 | ATCGGGAATTAGTCAAGGATAAAGAA | GACTGCTGATTGTACGTTCATATTCA |
| 33 | GAAGTTGACCTGGAACGCCA | CATTCATACCTTTTGTGCCGC |
| 34 | CACGGGCAACTTGCAAATC | GCCCCCCAAACATACCAAAT |
| 35 | ATGAACTGAGGCTTCGATTGC | TTGGTTCCATCTCTTTTCTGCC |
| 36 | TGTGAAGAAACATGAGGAAGACCTT | CCGTTTGTTTGAATTTATTTAGTGAA |
| 37 | AACAAGCATTTTATTCGTCTGGC | TTTGACCAAGAGTGAGGAAAGAGAG |
| 38 | AGAGGATTTAAAGTATCTTCTGGACCA | GCTTCTTTTTGAGCCTGAAGTTCA |
| 39 | CGGGCACTTTTAGAACTCCG | GGCCTCTTTTTGAGAAGTTGCA |
| 40 | TGCAAAAGAAACAAAAAGCCTATAATA | GCCTTTTCAGTTCATCATTCTCTTG |
| 41 | TAAACAAAGTCTAATTGAAGAACTCCA | TCTACTTCCTCCACCTTTCCCTCT |
| 42 | CTGGTAATTTTCCTACCTCCTTCG | CCACTTTTTACCTTCTTCCCACC |
| 43 | AAGAGAAACTTACTTTGCAGAGGAAAC | ACTCCAAAGCTCGTATTCCCAA |
| 44 | CAAGCTCTTCCTCGAGATTCTGTT | TTCTAAAGCATGAAGTTTTTCTTGGA |
| 45 | TGAAGATTTCAGGAATAGAGTCAGATG | CAGATGACAACTTCAAGTTTTCCTTC |
| 46 | TCAGAGATTTGAAGGAAATGTGTGA | CCCCTCTAACATGGCCAAGTT |
| 47 | GTGGAAAGACAATCCCAGAACTG | GTTCATTTTCTCTCTGGACTTTTTCA |
| 48 | ATCTTGGGCATCAGTTGAGCAT | GAAGCCTTTCATTTTCAGCAATAAT |
| 49 | TGAGAAGATGACAGTTCAACTAGAAG | GCACCTTCAAGCTGTGGACC |
| 50 | ACAATGAAGACCTTGAACAACAGG | GAACATCTTGCGATATTATGCATTAT |
| 51 | ATTTCCATGGCAAACCTTATCTTC | TCAGCACCTTCAGGAACATGTT |
| 52 | TCAGCTGGATAAAGAGAAAGCAGAA | GGTATGGTGCTTTCAGCTCCAC |
| 53 | ACCTTGAACTCATTCGTAGTGGG | TTTCCTTTAGTTGATCAGCATCTGTT |
| 54 | TCCCCATTTACTAAAGGTCACCTATAA | GTACAAGGTAGTGAGAAGGAAATAC |