ADM is elevated in lungs of mice exposed to CS. (A) Expression of ADM mRNA is elevated in lungs of mice exposed to CS compared to animals exposed to normal air. Strong ADM (B, C) and AHR (D, E) immunoreactivity colocalized in the epithelium of bronchioles of mice exposed to CS (x100). ADM expression was also elevated in the vascular endothelium of the lung in mice upon exposure to CS (F), although AHR was undetectable in the same vessels (G) (x600). In mice exposed to normal air, ADM immunoreactivity was localized exclusively to some muscle fibers around the bronchioles (H, I) and no AHR staining was detected (J, K) (x100).