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. Author manuscript; available in PMC: 2015 Feb 25.
Published in final edited form as: Cancer Res. 2012 Sep 19;72(22):5790–5800. doi: 10.1158/0008-5472.CAN-12-0818

Figure 3.

Figure 3

AHR activation results in induction of ADM expression in vitro and in vivo. (A) Increased ADM mRNA and protein was observed in A549 cells after exposure to 3MC for 24h. (B) Exposure of cancer cells from different anatomical origins to TCDD resulted in enhanced ADM mRNA levels, (C) which was found to be time dependent in breast and lung cells lines. (D) AHR null fibroblasts showed no increase in ADM transactivation after exposure to CSE (0.06 puffs/ml for 24h) as opposed to isogenic AHR+/+ cells. A marked increase in basal ADM transactivation was observed between untreated AHR+/+ and AHR−/− cells. (E) Forced co-expression of AHR and ARNT was required in A549 cells to induce upregulation of ADM mRNA. Consistently, transient transfection of two different siRNA for AHR resulted in significant blockage of ADM expression. (F) AHR-ARNT dependent stimulation of ADM expression was shown to be time dependent. (G) Low ADM transactivation driven by the complete ADM promoter (pGL3P-AM2725) in AHR null cells was restored by ectopic expression of AHR in a dose dependent manner.