Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Cells were cultured in leucine-free and serum-free media for 16 h and then stimulated with 10% FBS and with or without leucine for 2 h. Western blotting was performed for p-Akt (T308), p-Akt (S473), Akt, p-S6 (S235/236), p-S6K1 (T389), S6K1, p-4E-BP1 (T70), p-4E-BP1 (T37/46), p-4E-BP1 (S65), 4E-BP1, Non p-4E-BP1, eIF4E, p-eIF4G (S1108) and β-actin. The relative p-S6K1 (T389)/total-S6K1 and p-4E-BP1/total 4E-BP1 ratios are given. A: MDA-MB-468 cell line, B: MCF7 cell line.
