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Cells were grown with (0.5 mM) and without leucine in the presence or absence of 10% FBS. Western blotting was performed for p-Akt (T308), p-Akt (S473), Akt, p-S6 (S235/236), p-S6 (S240/244), p-S6K1 (T389), S6K1, p-4E-BP1 (T70), 4E-BP1, Non p-4E-BP1 and β-actin. The relative p-S6K1 (T389)/total-S6K1 and p-4E-BP1/total 4E-BP1 ratios are given. A: MDA-MB-468 cell line, B: MCF7 cell line.
