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. 2015 Feb 12;15:46. doi: 10.1186/s12870-015-0440-6

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© Gouthu and Deluc; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Hormone level of abscisic acid (ABA) (A), and the expression levels of ABA biosynthesis gene, NCED4 (B) and ABA-responsive gene, MYBa1 (C) in the pericarp of low and high seed weight-to-berry weight (SB) groups. ABA levels were quantified by LC-MS/MS using four replicates. Gene expressions were measured by qRT-PCR and expression levels are relative to those of low-SB berries at 2-wk PV (n = 5). Error bars indicate ± SEM. Prevéraison stages were two- and one-week before véraison (2-wk PV and 1-wk PV). Gene expressions were also measured at mid-véraison (MV) cluster stage. Significant differences between low- and high-SB berries at each cluster stage are indicated by asterisks (Student’s t-test, p < 0.05). Significant differences of each SB group between the ripening stages are denoted by different letters (lower and upper case letters are used for low- and high-SB berries, respectively (Tukey’s HSD, p < 0.05)).