Fig. 3.
αT-cat loss enhances the severity of TDI asthma. A: murine model for TDI asthma with sensitization by intranasal injection, challenge by nebulization and assessment by airway hyperresponsiveness (AHR) and bronchoalveolar lavage (BAL). B: whole-body plethysmography assessment of AHR. WT mice showed no increase in enhanced pause (Penh) AHR when exposed to TDI, whereas KO mice demonstrated a significant increase in Penh after TDI exposure. D: analysis of airway inflammation by BAL. TDI exposure increases macrophage and neutrophil infiltration similarly in WT and KO mice. Mϕ, macrophage, LY, lymphocyte, NE, neutrophil, EO, eosinophil. E: by hematoxylin and eosin staining, the small airways (AW) and pulmonary arteries (PA) of WT and KO mice exposed to TDI show no significant differences. F: in larger AW, WT and KO mice show adjacent pulmonary veins (PV). *P < 0.05, **P < 0.01, ***P < 0.001, by Student's t-test. Error bars represent SE.