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Acetoin production (in mM) by E. coli MG1655 containing pTrc99A-Ptrc-budAB or E. coli MG1655 ΔhycE containing pTrc99A-Ptrc-budAB during fermentative growth in LB with 5 g/l glucose, 1 mM IPTG, and 100 μg/ml ampicillin (Ap) for 48 h.
| Time (h) | E. coli MG1655 pTrc99A-Ptrc-budAB | E. coli MG1655 ΔhycE pTrc99A-Ptrc-budAB |
|---|---|---|
| 4 | 0.7 ± 0.2 | 0.5 ± 0.2 |
| 6 | 5.3 ± 0.7 | 5.0 ± 0.4 |
| 8 | 9.8 ± 0.9 | 7.1 ± 1.3 |
| 10 | 21.8 ± 0.8 | 18.4 ± 0.5 |
| 12 | 19.4 ± 1.2 | 12.5 ± 1.1 |
| 24 | 18.7 ± 0.7 | 12.3 ± 0.5 |
| 48 | 20.5 ± 2.3 | 11.1 ± 2.3 |
