Figure 1.

Effect of H₂O₂ on HUC-F2 cells. (A) HUC-F2 cells were treated with 0–1000 μM H₂O₂ incubated in 5% CO₂ for 48 hours, and viability was assessed with the MTT colourimetric assay, as described in Experimental Methods. High concentrations of H₂O₂ inhibited HUC-F2 cell growth. (B) NR4A1 mRNA levels were determined with real-time PCR in HUC-F2 cells treated with 0–300 μM H₂O₂ for 4 hours. Results are presented as mean ± standard deviation of three samples in each treatment group. **P < 0.01 versus control. Expression of NR4A1 was increased by H₂O₂ in a concentration-dependent manner. The differences between the groups were analyzed by ANOVA with Fisher's PLSD post hoc method.