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P0(P1-P2) 2 is a component of the OsSec18 complex in vivo. A, The P0(P1-P2)2 complex and OsSec18 are present in the same complex based on a gel-filtration experiment. The crude protein extract from rice immature endosperm was loaded on a Sepharose 300 gel filtration column and detected via Western blot using anti-Sec18 or anti-60sP0 serum; B, Co-IP of Os60sP0p and OsSec18 in stem, leaf and immature seed.
