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. 2015 Mar;56(3):515–525. doi: 10.1194/jlr.M053678

Fig. 6.

Fig. 6.

mRNA levels of TG biosynthetic pathway enzymes and DGAT activity. A: Control and CETP+ cells were incubated without or with 200 µM oleate for 48 h, then mRNA levels of GPAM (glycerol-3-phosphate acyltransferase 1, mitochondrial), LPIN1 (phosphatidate phosphatase), and DGAT1 and 2 were measured by qPCR. Data are expressed relative to the gene levels in SW872 cells incubated without oleate. Values are the mean ± SE of the values determined on RNA isolated from two to three separate cell cultures. * P < 0.05 versus control; + P < 0.05 versus oleate-treated control. B: DGAT activity in isolated microsomes. Data are the mean ± SD of values determined on two separate microsomal preparations for each cell type, each assayed in triplicate. * P < 0.05 versus all other values.