TABLE 1.
Cell | TG (nmol/mg Protein) | % of Vector Pool |
Control pool | 80.4 ± 9.5 | 100 |
Control clone 1 | 85.8 ± 17.3 | 106.7 |
Control clone 2 | 80.0 ± 2.0 | 99.5 |
Control clone 3 | 86.5 ± 10.4 | 107.6 |
CETP+ clone 2 | 47.2 ± 3.0 | 58.7 |
CETP+ clone 4 | 48.9 ± 6.8 | 60.8 |
CETP+ clone 9 | 55.3 ± 9.0 | 68.8 |
Vector-transfected SW872 cells (pool and individual clones) and three CETP+ clones were incubated with 200 µM 3H-oleate/BSA for 48 h to uniformly label the TG pool. TG was extracted, purified by thin layer chromatography, and quantified by scintillation counting. Values are mean ± SD, n = 3. The TG content of all CETP+ clones differed significantly from control (P < 0.05).