Fig. 2.
PI binds to RSV with high affinity. Aliquots of 1.5 nmol phospholipid (PI, POPG, or POPC) were adsorbed onto microtiter wells, which were subsequently blocked with 3% albumin in binding buffer. Varying concentrations of RSV (0.05–2.5 × 107 pfu/ml) were added to the wells and incubated at 37°C for 2 h. The wells were washed three times, and viral binding was detected by ELISA using an HRP-conjugated polyclonal antibody and quantified by spectrophotometry at 450 nm. Values shown are means ± SE for three independent experiments.