Fig. 1.

Effect of SM depletion by myriocin on ABCB4- and ABCA1-dependent lipid efflux from HEK293 cells. A: HEK293 cells were incubated in the absence or presence of 20 μM myriocin for 24 h. Cellular lipids were extracted, and SM content was analyzed. B: HEK/ABCB4 cells were pretreated with 0 or 20 μM myriocin for 24 h. Then, cells were incubated in the absence (empty bars) or presence (blue bars) of 1 mM NaTC for 24 h. Lipids in the medium were extracted, and PC content was analyzed. C–F: HEK/ABCA1 cells were pretreated with 0 or 20 μM myriocin for 24 h. Then, cells were incubated in the absence (empty bars) or presence (red bars) of 10 μg/ml apoA-I (C, D) or 1 mM NaTC (E, F) for 24 h. Lipids in the medium were extracted, and PC (C, E) and cholesterol (D, F) contents were analyzed.