Fig. 4.

Different distribution of ABCB4 and ABCA1 in detergent-soluble and detergent-insoluble fractions. BHK/ABCB4 (A) and BHK/ABCA1 (B) cells were solubilized with 1% Triton X-100, 1% Lubrol WX, or 1% CHAPS. Proteins from the soluble (S) and insoluble (IS) fractions were separated on 5–20% gradient SDS polyacrylamide gels, and ABCB4 and ABCA1 were immunodetected. Caveolin was detected as a marker for detergent-insoluble fractions. OptiPrep gradient fractions were separated on 5–20% gradient SDS polyacrylamide gels. C: ABCB4 and caveolin-1 were detected with specific antibodies.