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. 2014 Nov 10;10(2):527–538. doi: 10.1021/cb500689g

Figure 1.

Figure 1

Surface display and secretion constructs. (a) In display construct pCT4Re, Aga2p is expressed at the carboxy-terminus to anchor the fusion protein to the yeast surface, while a FLAG epitope tag is expressed on the amino-terminus of the scFv or GFP to indicate full-length construct expression on the yeast surface. In the intein-containing display constructs, the non-self-cleaving Mxe GyrA intein is inserted between the carboxy-terminus of the scFv or GFP and the Aga2p surface anchor. (b) Secretion construct pRS316-FLAG is similar to the surface display construct, with a synthetic prepro leader sequence directing secretion and a six histidine epitope for purification.