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. 2014 Nov 10;10(2):527–538. doi: 10.1021/cb500689g

Table 1. Intein Mutations and Surface Display Levels.

  amino acid
    
  21 33 50 51 74 105 107 110 112 114 117 118 124 129 144 158 160 164 168 190 191 fold increasea statistical significanceb
wild-type I I L F N I R F V C F A F Y H D R A S I T 1.0 ± 0.0  
202–03         D         R         R G           1.4 ± 0.1 **
202–08       L   V C S     L   L           G T   1.8 ± 0.2 **
202–12   T P           A   C                     1.5 ± 0.3 *
202–13 T     S           G   T         Q         1.3 ± 0.2 NS
505–05 T                 R                   V   1.3 ± 0.1 **
505–11                           C       P     M 1.7 ± 0.1 **
a

Fold increase relative to the wild-type intein as fusions to 4–4–20, mean ± SD from three independent yeast transformants.

b

Statistical analysis was perfomed by an unpaired Student’s t-test, with double asterisks representing p < 0.01, single asterisks representing p < 0.05, and NS designating that differences are not significant (p > 0.05).