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. 2015 Feb 19;10:1479–1492. doi: 10.2147/IJN.S74158

Figure 7.

Figure 7

NP uptake enhances apoptosis required for myoblast fusion.

Notes: (A) Representative annexin V-PE/7AAD labeling pattern of myoblast populations in HS medium 24 hours (h) after NP exposure or not (control), in the absence and the presence of calcium. The population positive for annexin V and negative for 7AAD (Q4) was used to quantify PS exposure at cell surface. (B) Exposed myoblast population positive for annexin V analyzed after 6 h in HS, 24 h in HS, and 24 h in FBS, followed by 24 h in HS. (C) Percentage of cells positive for cleaved caspase-3 analyzed after 24 h in HS and 24 h in FBS, followed by 24 h in HS. Data are expressed as mean ± standard error of the mean from three independent experiments (**P<0.01).

Abbreviations: NP, silica nanoparticle; HS, horse serum; FBS, fetal bovine serum; 7AAD, 7-amino-actinomycin D; PE, phycoerythrin; PS, phosphatidylserine.