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. 2014 Nov 25;290(3):1404–1411. doi: 10.1074/jbc.M114.622761

FIGURE 4.

FIGURE 4.

Knockdown of MCM7 or SF3B3 or interference of MCM7/SF3B3 interaction resulted in cell death. A, immunoblotting of SF3B3, MCM7, and GAPDH in PC3 or DU145 cells treated with indicated siRNA. PC3 or DU145 cells (1 × 106) were transfected with 80 pmol of siMCM7, siSF3B3, or siScramble (Scr). These cells were harvested for analysis 24 h after the transfection. B, annexin V and propidium iodide staining of PC3 or DU145 cells treated with siRNA as in A to detect apoptosis and necrotic cell death. C, immunoblotting of ΔMCM7aa1,221–248-FLAG and GAPDH in Pmotif#1 and Pmotif#2 cells induced with or without tetracycline (5 μg/ml) for 24 h using antibodies specific for FLAG and GAPDH. D, annexin V and propidium iodide staining of Pmotif#1 and Pmotif#2 cells as in C to detect apoptosis and necrotic cell death.