FIGURE 3.

Histamine-induced vascular hyperpermeability affects viral sequestration patterns. BALB/c mice (n = 4) were treated with AAV9 or AAV9/NGA mutant virions alone or coinjected with histamine to concurrently induce vascular hyperpermeability. Spleen (A–D) and liver (E–H) tissue sections were immunostained with the ADK9 mouse monoclonal antibody against AAV9 capsids followed by an Alexa Fluor 647 goat anti-mouse secondary antibody (red). Immunostained tissue sections were mounted with ProLong Gold antifade reagent with×63 oil objective. Representative micrographs obtained from multiple tissue samples are shown.