FIGURE 4.

Colocalization of Ca_v1.4 α₁ with β₂ or α₂δ₄ at the photoreceptor synaptic ribbon. Mouse retina sections were double-labeled with anti-Ca_v1.4 α₁ (green) and anti-α₂δ₄ (red) (A) or anti-β₂ (red) (B). The overlay is shown in the right panel together with Hoechst staining. Higher magnification images of the OPL are shown in the bottom panels. Ca_v1.4 shows a high degree of colocalization with β₂ and α₂δ₄, as indicated by the calculated Pearson's correlation coefficient between the Alexa 488-stained Ca_v1.4 and the Alexa 555-stained β₂ (r ≥ 0.710) or α₂δ₄ (r ≥ 0.608). OS, outer segment; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer.