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. 2014 Dec 2;290(3):1505–1521. doi: 10.1074/jbc.M114.607465

FIGURE 5.

FIGURE 5.

Isolation of Cav1.4 α11-β2-α2δ4 complexes from cotransfected HEK293 cells and mouse retina. A and B, Cav1.4 α1 interacts with β2X13, α2δ4, and CaBP4 expressed in HEK293 cells. Coimmunoprecipitations were performed with control mouse IgG or anti-FLAG antibody using lysates of HEK293 cells untransfected (UC) or cotransfected (TC) with β2X13, α2δ4, and CaBP4 with or without (no Cav1.4) FLAG-Cav1.4 α1. Experiments were performed in the presence (A) or in the absence of 0.1 mm Ca2+ (B). Western blotting (IB) was with anti-FLAG, anti-β2, anti-α2δ4, or anti-CaBP4. Left panels show input lysates. Right panels show coimmunoprecipitated (IP) proteins. Results shown are representative of three experiments. C, Cav1.4 α1 interacts with β2 and α2δ4 in mouse retina. Coimmunoprecipitations were performed using lysates from WT or KO Cav1.4 α1 mouse retina with anti-α2δ4 or anti-β2 antibodies. Anti-Cav1.4 α1 antibody was used for Western blotting. 8% of lysate was used for input.