TABLE 1.
-Fold difference in transcript levels of β2X13 and β2a in human retina
qPCR data in Fig. 1B were analyzed using the 2−ΔΔCt as described by Livak et al. (34). -Fold differences in Ct values between β2X13 and β2a were calculated after adjustment to GAPDH control and are presented as mean ± S.D. (n = 3).
| cDNA | β2a ΔCt (mean β2a Ct − mean GAPDH Ct) | β2×13 ΔCt (mean β2×13 Ct − mean GAPDH Ct) | ΔΔCt (mean β2×13 ΔCt − mean β2a ΔCt) | Normalized β2×13 RNA amount relative to β2a RNA or 2−ΔΔCt |
|---|---|---|---|---|
| cDNA1 | 13.36 ± 1.04 | 9.51 ± 1.09 | −3.85 ± 1.09 | 14.45 ± 10.5 |
| cDNA2 | 11.15 ± 0.28 | 9.55 ± 0.55 | −3.15 ± 0.55 | 8.9 ± 3.4 |
| cDNA3 | 11.96 ± 2.00 | 5.69 ± 0.47 | −6.27 ± 0.47 | 77.5 ± 25.1 |