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. 2014 Nov 19;290(3):1546–1558. doi: 10.1074/jbc.M114.579565

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FIGURE 5. — Chronic elevation of NAD in muscle does not alter the NAD/NADH ratio. NAM (A), NMN (B), and NADH (C) content of quadriceps muscle in 5-month-old mice (n = 4–11 mice per group). D, measured ratio of total NAD/NADH in quadriceps muscle. NAD values were derived from Fig. 1D. Shown are the measured ratio of lactate/pyruvate (E) and β-hydroxybutyrate/acetoacetate (BHB/Acac) (F) in quadriceps (n = 3–7 per group). Shown is the calculated free NAD/NADH ratio in the nucleocytosolic (G) and mitochondrial (H) compartments. Error bars represent S.E. *, p < 0.05; ***, p < 0.001 by one-way analysis of variance with Bonferroni's multiple comparisons test.