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. 2014 Dec 1;290(3):1580–1591. doi: 10.1074/jbc.M114.609834

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FIGURE 4. — BAP1 down-regulates FoxK2 target genes. A, expression levels of FoxK2 target genes in BAP1-depleted H1299 cells. mRNA levels of the indicated FoxK2 target genes were measured by RT-PCR in control and BAP1-depleted H1299 cells (right panel). Knockdown of BAP1 was assessed by Western blotting (left panel). β-Actin was used as a loading control. B, effects of various mutations in BAP1 on expression of FoxK2 target genes. The indicated BAP1 mutants were expressed in the H226 BAP1-deficient cell line as in Fig. 3B, and expression of the indicated genes was quantitated by RT-PCR. C, expression of FoxK2 target genes in FoxK2-depleted H1299 cells. FoxK2 was depleted with the indicated siRNAs, and FoxK2 protein levels were assessed by Western blotting (left panel). An asterisk indicates a nonspecific band. mRNA levels of the indicated genes were measured by RT-PCR (right panel). qPCR was done in triplicate, and mean ± S.D. is shown. Values were normalized to GAPDH and are shown relative to control samples.