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Field dependence of the 31P PR1E for diC8PI and diC8PI(4,5)P2 in the presence of spin-labeled PTEN C124S. A, effect of 4.3 μm spin-labeled C124S on 3 mm diC8PI(4,5)P2: P-1 (●); P-4 (□); (▴) P-5. B, comparison of PRE for diC8PI (3 mm) produced by spin-labeled 2.0 μm native PTEN (○) or 2.2 μm spin-labeled variant C124S (●); the field dependence was fit with τP-e = 200 ns, and the arrow approximates the amount of R1 contributed by a spin label on Cys-124.
