FIGURE 2.
Gene expression array analysis performed in human A375 malignant melanoma cells exposed to aurin or celastrol. A, the scatter blot depicts differential gene expression as detected by the RT2 Human Stress and Toxicity ProfilerTM PCR Expression Array technology (aurin, 10 μm, 6 h exposure). Upper and lower lines, cut-off indicating 4-fold up- or down-regulated expression, respectively. Arrays were performed in three independent repeats and analyzed using the two-sided Student's t test. B, numerical expression changes (p < 0.05) elicited by aurin. C, for comparison, an analogous experiment was performed using the established heat shock response inducer celastrol (10 μm, 6-h exposure), and celastrol-induced numerical expression changes are listed (p < 0.05).