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kd of ScThrRS for SctRNAThr (AGU) in filter binding reaction at 4 °C
| Enzyme | kda |
|---|---|
| μm | |
| WT | 3.3 ± 0.3 |
| R52F | 3.0 ± 0.2 |
| R52D | 3.1 ± 0.5 |
| G145D | 3.0 ± 0.8 |
| G356N | 13 ± 3 |
| S308F | 3.0 ± 0.3 |
| S457F | 3.8 ± 0.5 |
| G458D | 3.1 ± 0.5 |
| R461H | 3.2 ± 0.5 |
| P627L | 19 ± 5 |
| G685D | 3.9 ± 0.9 |
a The results are shown as the average of three independent trials ± S.D.
