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kobs of AMP formation of ScThrRS and derivatives in the presence of Ser
| Enzyme | tRNA | kobsa | Relative kobs |
|---|---|---|---|
| ×10−3 s−1 | % | ||
| WT | − | 2.8 ± 0.8 | 18.9 |
| + | 14.9 ± 0.8 | 100 | |
| R52F | − | 0.3 ± 0.1 | 2.1 |
| + | 0.6 ± 0.2 | 4.0 | |
| G145D | − | 3.1 ± 0.2 | 20.6 |
| + | 4.1 ± 0.4 | 27.5 | |
| S308F | − | 0.5 ± 0.1 | 3.4 |
| + | 2.9 ± 0.4 | 19.4 | |
| C-Δ3 | − | 1.4 ± 0.1 | 9.3 |
| + | 13.4 ± 0.9 | 89.9 | |
| C-Δ5 | − | 1.1 ± 0.2 | 7.1 |
| + | 8.2 ± 0.4 | 54.9 | |
| C-Δ10 | − | 0.9 ± 0.2 | 6.2 |
| + | 7.7 ± 0.5 | 51.7 |
a The results are shown as the average of three independent trials ± S.D.
