FIGURE 5.

Metabolic stability of unstapled versus stapled peptide in human blood plasma and in bovine liver homogenates. 10 μm peptides (TAMRA-labeled TM4(88–100) and TAMRA-labeled S-TM4(88–100)-N95) were incubated in the respective medium for the indicated time periods. Cleavage products were separated by reversed phase HPLC at 565 nm and identified by MALDI-TOF mass spectrometry. Decay of the intact peptide (%) was determined by peak integration. See ”Materials and Methods“ for further details.