TABLE 2.

Kinetic parameters for the substrates and inhibition constants of AC4-AC10 for the reaction of the native SBG

Substrate	Kinetic parametersa			Inhibition constants
	kcat	Km	kcat/Km	Inhibitor	Kib	Type
					μm
Glc2	384 ± 4	17.8 ± 0.9	21.6
Glc3	442 ± 2	2.91 ± 0.07	152
Glc4	388 ± 5	1.74 ± 0.03	223	AC4	15.4 ± 3.5	Competitive
Glc5	478 ± 2	0.623 ± 0.017	767	AC5	2.66 ± 0.21	Competitive
Glc6	464 ± 2	0.359 ± 0.004	1,290	AC6	1.63 ± 0.73	Competitive
Glc7	457 ± 1	0.327 ± 0.002	1,400	AC7	0.845 ± 0.160	Competitive
Glc18	436 ± 4	0.293 ± 0.010	1,490	AC8	0.888 ± 0.032	Competitive
Soluble starchc	405 ± 3	0.166 ± 0.002	2,440	AC9	0.936 ± 0.177	Competitive
				AC10	1.01 ± 0.13	Competitive

^a k_cat, s⁻¹; K_m, mm; k_cat/K_m, s⁻¹ mm⁻¹. Each k_cat or K_m with standard deviation is the average value of the triplicate measurements.

^b Each K_i with standard deviation is average value at three concentrations of inhibitor.

^c The K_m value for soluble starch is its concentration of non-reducing termini.