Figure 4.

Voriconazole has little effect on mGuR6-mediated activation of GIRK currents. (A) Patch-clamp recordings of CHO cells expressing mGluR6-EYFP and GIRK potassium channels demonstrated that an mGluR6-coupled GIRK current could be activated by application of 1 mM glutamate in a high-potassium (High K) external solution. Only a very slight decrease in the current was observed when the glutamate solution was replaced by glutamate plus voriconazole (100 μM). Return to glutamate led to a slight increase in the current. The effect of voriconazole on mGluR6 is mild. Vertical spikes represent current elicited in response to voltage ramps. (B) Individual currents in Ringer's solution (control), High-potassium solution (High K), 1 mM glutamate in high-potassium solution (Glu), and 100 μM voriconazole in 1 mM glutamate high-potassium solution (Glu + Voricon) were recorded during the voltage ramp and plotted versus voltage, showing that voriconazole has only a minor effect on GIRK currents evoked by mGluR6 receptor activation.