Figure 8.

Relative ROS levels for CHO, B16, and MYO cells incubated with increasing concentrations of PAA-coated NPs for 24 and 48 hours as spectrofluorimetrically determined by CM-H₂DCFDA assay.

Notes: NC represents untreated cells, while cells in PC were exposed to 500 μM H₂O₂ for 1 hour. Values are presented as a percentage of ROS in treated cells compared to negative control. Mean and standard error are shown for three independent experiments.

Abbreviations: B16, mouse melanoma cell line; CHO, Chinese Hamster Ovary cell line; CM-H₂DCFDA, 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate; MYO, primary human myoblasts; NC, negative control; NP, nanoparticle; PAA, polyacrylic acid; PC, positive control; ROS, reactive oxygen species.