Table 2.
Vaccinia Fluorescent Reporter Viruses
| Category | Virus | Promoter | Fluorophore |
|---|---|---|---|
| Vaccinia fluorescent reporter viruses | |||
| Single-stage reporter | Early Venus (EV) | C11R | Venus |
| Intermediate Venus (IV) | G8R | Venus | |
| Late Venus (LV) | F17R | Venus | |
| Late Red (LR) | F17R | mCherry | |
| A4L-Venus | A4L endogenous | Venus-p39 fusion protein | |
| A4L-mCherry | A4L endogenous | mCherry-p39 fusion protein | |
| Multi-stage reporters | Late Red, Early Venus (LREV) | C11R, F17R | Venus (Early), mCherry (Late) |
| Intermediate Red, Early Venus (IREV) | G8R, F17R | Venus (Early), mCherry (Intermediate) | |
| Triple Virus (TrpV) | C11R, G8R, F17R | Venus (Early), mCherry (Intermediate), TagBFP (Late) | |
| Control | Promoter-less Venus (PLV) | No promoter | Venus (not expressed) |
VACV created to monitor viral infection by fluorescent reporter, with promoter(s) and fluorophore(s) listed.
VACV, vaccinia virus.