Skip to main content
. Author manuscript; available in PMC: 2016 Jan 6.
Published in final edited form as: Cell Metab. 2015 Jan 6;21(1):138–149. doi: 10.1016/j.cmet.2014.12.014

Figure 5. Overexpression and shRNA-mediated knock down of selected genes in 3T3-L1 adipocytes.

Figure 5

Selected genes from the set of 30 species conserved and T2D-SNP overlapping adipose DMRs were either stably overexpressed (A) or knocked down with shRNA (B). Glucose uptake is plotted as fold difference from normal, and significance was determined by two-way ANOVA modified by Bonferroni correction denoted as follows: * p<0.05, ** p<0.01, *** p<0.001. (C) DNA methylation and gene expression levels for high-fat-fed mice and obese human versus low-fat-fed mice and lean humans (e.g., “↓“ indicates hypomethylation / lower gene expression in high-fat-fed and obese compared to low-fat-fed and lean). Bold arrows indicate significant changes.