Table I. Analysis of selective cell surface markers on resting and differentiated U937 cell clonesa.
| U9337 Cell Clone |
|||||
|---|---|---|---|---|---|
| Marker | Treatment | WT | CMV | 36.4AS | 15S |
| ICAM-1 | None | 400 ± 20 | 380 ± 20 | 370 ± 15 | 410 ± 20 |
| ICAM-1 | PMA | 1250 ± 50 | 1400 ± 150 | 1200 ± 200 | 1300 ± 100 |
| PECAM-1 | None | 450 ± 20 | 470 ± 30 | 380 ± 30 | 500 ± 50 |
| PECAM-1 | PMA | 600 ± 20 | 700 ± 50 | 500 ± 40 | 650 ± 40 |
| CD11a | None | 410 ± 10 | 400 ± 20 | 400 ± 10 | 400 ± 15 |
| CD11a | PMA | 1000 ± 50 | 1100 ± 50 | 1050 ± 50 | 1000 ± 30 |
| CD11b | None | 40 ± 5 | 42 ± 4 | 38 ± 5 | 45 ± 5 |
| CD11b | PMA | 200 ± 15 | 205 ± 15 | 210 ± 5 | 200 ± 5 |
| CD11c | None | 85 ± 5 | 70 ± 10 | 75 ± 5 | 88 ± 4 |
| CD11c | PMA | 215 ± 15 | 230 ± 20 | 200 ± 10 | 235 ± 25 |
| CD49d | None | 11 ± 3 | 13 ± 3 | 13 ± 2 | 20 ± 10 |
| CD49d | PMA | 90 ± 16 | 81 ± 9 | 74 ± 13 | 75 ± 5 |
| CXCR4 | None | 1250 ± 100 | 800 ± 20 | 830 ± 30 | 850 ± 50 |
| CXCR4 | PMA | 250 ± 20 | 300 ± 30 | 250 ± 15 | 280 ± 20 |
a
Values (mean ± SEM) are in median fluorescence intensity units of n = 4 determinations. Cell incubation with PMA (6 ng/ml, 48 h) significantly up-regulated adhesion molecule expression and down-regulated CXCR4 levels, and this occurred equally in all clones.